Post by Admin on Feb 3, 2019 12:05:46 GMT
Extraction of cannabinoids from cannabis sativa L plant and its potential antimicrobial activity
Drug resistance of microorganisms is a big threat for the human health. In order to overcome this
problem an alternative potential treatment is the use of herbal medicines. Plants are not only
considered as dietery supplements to living organisms but also tradionally used for treating many
diseases and no sign of drug resistance has been reported till now. The plant leaves were identified as
Cannabis sativa L. The cannabinoids were extracted by aqueous extraction found a total yield of 3.8g
while as acetone extract 4.8g. The protein content in crude extract of Cannabis sativa L. for aqueous
extract was found 112µg/ml and for acetone extracts 160µg/ml. The molecular weight of protein by SDS
PGAGE was found to be 70KDa. The HPLC intension percentage for aqueous extract was 11 while for
acetone extract it was found 25. The acetone extract exhibited higher antimicrobial activity by showing
zone of inhibition against bacteria Pseudomona aeruginosa, Vibro cholerae and fungi Cryptococcus
neoforms, Candida albicans. The cannabiniods extracts showed the antioxidant activity by changing to
yellow colour.
Source: www.theroc.us/researchlibrary/Extraction%20of%20cannabinoids%20from%20cannabis%20sativa%20L%20plant%20and%20its%20potential%20antimicrobial%20activity.pdf
Drug resistance of microorganisms is a big threat for the human health. In order to overcome this
problem an alternative potential treatment is the use of herbal medicines. Plants are not only
considered as dietery supplements to living organisms but also tradionally used for treating many
diseases and no sign of drug resistance has been reported till now. The plant leaves were identified as
Cannabis sativa L. The cannabinoids were extracted by aqueous extraction found a total yield of 3.8g
while as acetone extract 4.8g. The protein content in crude extract of Cannabis sativa L. for aqueous
extract was found 112µg/ml and for acetone extracts 160µg/ml. The molecular weight of protein by SDS
PGAGE was found to be 70KDa. The HPLC intension percentage for aqueous extract was 11 while for
acetone extract it was found 25. The acetone extract exhibited higher antimicrobial activity by showing
zone of inhibition against bacteria Pseudomona aeruginosa, Vibro cholerae and fungi Cryptococcus
neoforms, Candida albicans. The cannabiniods extracts showed the antioxidant activity by changing to
yellow colour.
Source: www.theroc.us/researchlibrary/Extraction%20of%20cannabinoids%20from%20cannabis%20sativa%20L%20plant%20and%20its%20potential%20antimicrobial%20activity.pdf