Post by Admin on Mar 11, 2019 20:02:22 GMT
Potentiating effect of β‐caryophyllene on anticancer activity of α‐humulene, isocaryophyllene and paclitaxel
Abstract
β‐caryophyllene is a sesquiterpene widely distributed in essential oils of various plants. Several biological activities are attributed to β‐caryophyllene, such as anti‐inflammatory, antibiotic, antioxidant, anticarcinogenic and local anaesthetic activities. In this work, the potentiating effect of β‐caryophyllene on the anticancer activity of α‐humulene, isocaryophyllene and paclitaxel against MCF‐7, DLD‐1 and L‐929 human tumour cell lines was evaluated. A non‐cytotoxic concentration of β‐caryophyllene significantly increased the anticancer activity of α‐humulene and isocaryophyllene on MCF‐7 cells: α‐humulene or isocaryophyllene alone (32 μg mL−1) inhibited cell growth by about 50% and 69%, respectively, compared with 75% and 90% when combined with 10 μg mL−1 β‐caryophyllene. Moreover, β‐caryophyllene potentiated the anticancer activity of paclitaxel on MCF‐7, DLD‐1 and L‐929 cell lines. The highest potentiating effect was obtained in DLD‐1 cells treated with paclitaxel combined with 10 μg mL−1 β‐caryophyllene, which increased the paclitaxel activity about 10‐fold. The intracellular accumulation of paclitaxel‐oregon green was evaluated in combination with concentrations of β‐caryophyllene ranging from 2.5 to 40 μg mL−1. β‐Caryophyllene (10 μg mL−1) significantly increased the intracellular accumulation of paclitaxel‐oregon green (about 64% over controls). Moreover, β‐caryophyllene induced intracellular accumulation of calcein but not verapamil, an inhibitor of P‐glycoprotein and multidrug resistance related protein transporters, suggesting that β‐caryophyllene promotes drug accumulation by a different mechanism of action. These results suggest that β‐caryophyllene facilitates the passage of paclitaxel through the membrane and thus potentiates its anticancer activity.
Source: onlinelibrary.wiley.com/doi/abs/10.1211/jpp.59.12.0005
Abstract
β‐caryophyllene is a sesquiterpene widely distributed in essential oils of various plants. Several biological activities are attributed to β‐caryophyllene, such as anti‐inflammatory, antibiotic, antioxidant, anticarcinogenic and local anaesthetic activities. In this work, the potentiating effect of β‐caryophyllene on the anticancer activity of α‐humulene, isocaryophyllene and paclitaxel against MCF‐7, DLD‐1 and L‐929 human tumour cell lines was evaluated. A non‐cytotoxic concentration of β‐caryophyllene significantly increased the anticancer activity of α‐humulene and isocaryophyllene on MCF‐7 cells: α‐humulene or isocaryophyllene alone (32 μg mL−1) inhibited cell growth by about 50% and 69%, respectively, compared with 75% and 90% when combined with 10 μg mL−1 β‐caryophyllene. Moreover, β‐caryophyllene potentiated the anticancer activity of paclitaxel on MCF‐7, DLD‐1 and L‐929 cell lines. The highest potentiating effect was obtained in DLD‐1 cells treated with paclitaxel combined with 10 μg mL−1 β‐caryophyllene, which increased the paclitaxel activity about 10‐fold. The intracellular accumulation of paclitaxel‐oregon green was evaluated in combination with concentrations of β‐caryophyllene ranging from 2.5 to 40 μg mL−1. β‐Caryophyllene (10 μg mL−1) significantly increased the intracellular accumulation of paclitaxel‐oregon green (about 64% over controls). Moreover, β‐caryophyllene induced intracellular accumulation of calcein but not verapamil, an inhibitor of P‐glycoprotein and multidrug resistance related protein transporters, suggesting that β‐caryophyllene promotes drug accumulation by a different mechanism of action. These results suggest that β‐caryophyllene facilitates the passage of paclitaxel through the membrane and thus potentiates its anticancer activity.
Source: onlinelibrary.wiley.com/doi/abs/10.1211/jpp.59.12.0005